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Objective To validate the analytical performance of a cardiac troponin I(cTnI)assay AccuTnI+3 on chemiluminescnet analyzer DXI800 and Access2;and to establish the 99th percentile of cTnI in an apparently healthy Chinese population.Methods The subjects are composed of 1 369 apparently healthy people and 20 acute myocardial infarction(AMI)patients from Wuhan Asian Heart Hospital and Fuwai Hospital from October 2014 to June 2015.The healthy people include 680 males and 689 females;with 340 subjects aged 18-30,674 subjects aged 31-64, and 355 subjects aged ≥65.The detection limits and imprecision of AccuTnI +3 assays were validated according to CLSI EP 15-A2 and EP17-A2 documents;the same samples were analyzed on DXI800 and Access2 to assess the consistency between the two analyzers using Bland Altman plot and Passing-Bablok regression.The correlation between different sample types (lithium heparin plasma, EDTA plasma & serum)were assessed using linear regression analysis.The lithium heparin plasmasamples from 1 369 apparently healthy people were analyzed to calculate the 99th percentile of cTnI.The cTnI concentrations were compared among age and sex groups.The 99th percentile of cTnI were also calculated for each group.The detection rate of cTnI in apparently healthy people was calculated using SPSS23.0.Results The limit of blank(LoB), limit of detection(LoD), and limit of quantification(LoQ)where CV%=10% were 0.007 ng/ml,0.010 ng/ml and 0.016 ng/ml on DXI800;0.008 ng/ml,0.012 ng/ml and 0.026 ng/ml on Access2,respectively.The cTnI measurements on DXI800 and Access2 were consistent and comparable.The cTnI concentrations of lithium heparin plasma, EDTA plasma and serum samples were linearly correlated pairwise: EDTA plasma measuremen t =0.76 heparin plasma measurement, R2=0.999(n=40, P<0.001); serum measuremen t =1.05 heparin plasma measurement,R2=0.996(n=40,P<0.001); serum measuremen t=1.38 EDTA plasma measurement, R2=0.993(n=40,P<0.001).The 99th percentiles were 0.030 ng/ml and 0.035 ng/ml on DXI800 and Access2,respectively,from 1 369 apparently healthy Chinese people.cTnI is significantly higher in elder group than in younger group.The 99th percentiles in 18-30 years old group,31-64 years old group,and≥65 years old group are:0.011 ng/ml,0.029 ng/ml,and 0.035 ng/ml respectively for DXI800;0.023 ng/ml,0.034 ng/ml, and 0.045 ng/ml respectively for Access2.cTnI is significantly higher in men than in women.The 99th percentiles in men and women are: 0.034 ng/ml and 0.032 ng/ml respectively for DXI800;0.043 ng/ml and 0.031 ng/ml respectively for Access2.cTnI was measurable in 62%and 87%of healthy subjects on DXI800 and Access2 systems,respectively.Conclusions The analytical performance of AccuTnI+3 assay fulfills the need of clinical use and the criteria of high-sensitive cardiac troponin assay.
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Objective To evaluate the value of the combination detection of serum amylase (AMY) and lipase(LPS) in the diag‐nose of acute pancreatitis .Methods Serum AMY and LPS were detected in 51 patients with acute pancreatitis ,418 patients with a‐cute abdomen disease and 78 healthy control subjects .Results The sensitivity of serum AMY and LPS increase in 51 patients with acute pancreatitis was 86 .3 % and 92 .2 % respectively .The specificity of serum AMY and LPS increase ,and both AMY and LAS increase in 418 non‐pancreatitis patients with acute abdomen disease was 90 .4% ,91 .7% and 100% respectively .Conclusion The combination detection of serum AMY and LPS can increase the sensitivity and specificity for diagnosing acute pancreatitis and has an important significance for rapid diagnosis of acute pancreatitis .
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Objective To evaluate the performance of Beckman Coulter Enhanced troponin Ⅰ immunoassay system (including the limit of detection and total imprecision) and establish the 99th percentile of Enhanced troponin Ⅰ in apparently healthy Chinese people in Kunming and Wuhan.Methods Evaluated the limit of detection and total imprecision of Beckman Enhanced troponin Ⅰ according to protocols EP of Clinical Laboratory Standards Institute; chose apparently healthy people from Wuhan (average altitude of 27 m) which represents plain regions,and Kunming (average altitude of 1895 m) which represents plateau regions.760 subjects from Wuhan were selected,aging from 30 to 91,included 400 males and 360 females.A total of 192 subjects from Kunming were selected,aging from 30 and 77,60 patients,which included 60 male and 132 female cases.To calculate the 99th percentile of Enhanced troponin Ⅰ by region,age,and gender.Results The limit of detection of Beckman Enhanced troponin Ⅰ is 0.013 μg/L,the cTnI concentration is 0.025 μg/L at 10％ CV.The 99th percentile of Enhanced troponin Ⅰ of the total population in Wuhan is 0.036 μg/L,the 99th percentile of men and women are 0.038 μg/L and 0.035 μg/L respectively,the 99th percentile in the 30-69 years group and over 70 years group are 0.038 μg/L and 0.035 μg/L respectively.The 99th percentile of Enhanced troponin Ⅰ of the total population in Kunming is 0.040 μg/L.To keep the 2nd digit after decimal point for results from Wuhan and Kunming,the 99th percentile of Enhanced troponin Ⅰ of the apparently healthy Chinese in 0.04 μg/L,where the CV is 8.23％.The percentage of positive samples detected below the 99th percentile in the normal reference population in Wuhan is 94％.Conclusions The 99th percentile of Beckman Enhanced troponin Ⅰ of the Chinese apparently healthy people is 0.04 μg/L,where the total imprecision is 8.23％,and the detection performance reach the acceptable levels per the guideline.
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Sino 0.5 or 2 mg/kg iv reduced significantly heart rate ( HR), systolic blood pressure ( SBP ), diastolic blood pressure ( DBP ), left ventricular systolic pressure ( LVSP) , dP/dtmax, cardiac index ( CI ) and total peripheral resistance ( TPR ) . VCE- + dP/dtmax was unchanged. T value did't change in 0 .5mg/kg, but it elevated in 2 mg/kg.
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It was reported that Os-thole(Ost) was isolated from the fruits of Cnidium monnieri(L. ) Cusson, possessesanti - arrhythmic action and depressed the aortic pressure action in animal studies. Its calcium -antagonistic effects were studied on the isolated rabbit aortic strips.Ost shifted the dose response curves for NE, CaCl2 and KC1 nonparallelly to the right, and depressed their maximal response; pD2' value is 3. 16, 4. 28 and 4. 39 respectively. It produced neither ?- a-drenoceptic stimulating nor ? - adreno-ceptic blocking effects. At the concentration of 100 ?mol ? L-1, Ost significantly inhibited the intra cellular Ca2+ dependent component of NE - induced contraction of the aortic strips, however, it didn't in-hibit the extra cellular Ca2+ dependent component of NE - induced contraction of the aortic strips, These results indicated that Ost produced relaxation on vascular smooth muscle might result from its antagonistic effect to Ca2+ and mainly blocked calcium influx through PDC, and the mode of its anta -gonism is similar to that of verapamil.
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The effects of metoclopramide( MCP ) on the physiologic properties of guinea pig papillary muscles and the hemodynamics in anesthetic dogs were studied. MCP decreased contractility, increased the epinephrine concentration inducing automaticity and shifted the voltage-time plot to the right. But the functional refractory period was prolonged. Furthermore, the contractility and automaticity of the right auricle were decreased. MCP 5 mg/kg iv increased cardiac index (CI), systolic blood pressure ( SBP ), diastolic blood pressure ( DBP ), left ventricular systolic pressure ( LVSP ), maximal rate of change of intraventricular pressure ( dP/dtmax ), and total peripheral resistance ( TPR ) while MCP 10mg/kg iv decreased SBP, DBP, LVSP, dP/dtmax and TPR except increase of CI.
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The effect of 4 -Phosphate- lydroxypiperaquine ( PHPQ) on the phsiologic properties in isolated auricle of guinea pig was studied.Twenty minutes after PHPQ 30 ?mol/L was added, the contractility & dT/dtmax of the left atria of guinea pig were decreased to 74.4 ? ( SD ) 5.3%(n=6, P
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The effects of dl-Tetrahydropalmatine ( THP ) on the physiologic properties in the auricle of guinea pig and on isoproterenol and calcium-mediated positive chronotropic action in guinea pig atria were studied.30 minutes after THP 52 nM was added, the contractility was decreased to 53+ ( SD ) 17%(P0.05) in the left auricle of guinea pig. Furthermore, the contractility and automaticity of the right auricle were decreased to 48+13% (P
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Dimethyltrilobine iodide (DMT) 1.5 M-M increased the contractile force of isolated guinea pig left atria. This positive inotropic action could not be an-tagonized by either propranolol, cimetidine or diphen-hydramine. DMT 1.5 nM also increased the contractile force of isolated guinea pig left atria exposed to low concentration of calcium or to verapamil.
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Metoclopramide ( MCP ) is similar to Procainamide in structure. In rats anaesthetized by ip chloral hydrate 300 mg/kg, arrhythmia induced by BaCl 3 mg/kg was shown to be antagonized by injecting iv MCP 30 mg/kg. It was also found that MCP 10 mg/kg injected iv protected rabbits against a'rrhythmia induced by chloroform-adrenaline . In anaesthetized rats, MCP 30 mg/kg injected iv raised the dose of aconitine required to induce first arrhythmia, ventricular fibrillation ( VF ) and heart arrest, while in anaesthetized guinea pigs, the same amount of MCP injected iv showed no significant action on the dose of ouabain inducing these effects. Ventricular tachycardia produced by occlusion of coronary artery in rats could not be prevnted by injecting iv MCP 30 mg/kg. It was found that MCP has local anaesthesia action.
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After Sinomenine 27 ?M was given, the contractibility was decreased to 51?11% (SD) and dT/dt max to 50?14% (SD). The automaticity induced by epinephrine was inhibited. Sinomenine (2.7-270 ?M) caused a concentration-dependent prolongation in the functional period. The amplitude of contraction and the heart rate in the right auricle was also inhibited. The results indicate that sinomenine may be antogonistic to both Ca~(++) influx and inward Na~(++) current.
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The effects of amrinone on the contraction and action potential of isolated guinca pig papillary muscles were studied with intracellular microelectrodes. When the preparations were exposed to 25 ?mol/L amrinone, their pcak developed tension(DT) was inereased by 65.4%, the maximum rate of force development (dT/dt_(max)) was increased by 50.8% the time to peak developed tension (tPT) was shortened by 15.9%. By raising the concentration of amrinone to 250 ?mol/L, DT and dT/dt_(max) were increased by 98.5% and 91.3%, respectively, and tPT was shortened by 24.7%. Concomitantly, amrinone can prolong the durations of action potential (APD50, APD90)and the effective refractory period (ERP) of the ventricular cells.These results suggest that amrinone (made in China) possesses the obvious positive inotropic effect.
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Osthole, the coumarin isolated from the fruits of Cnidum monnieri (L.) Cusson. The effects of osthole on the physiological properties in isolated auricle of guinea pig were studied. It possesses dose—dependent negative inotropic effects, the BaCl_2 inducing automaticity was inhibited, and the functional refractory period (FRP) was prolonged, and the frequence of sinoatrial node was slowed The negative inotropism and automatism suggest that sothole mey be antagonis tic to Ca~(2+), while the decrease of excitability and the prolongation of FRP indicate that it may be due to its inhibition on Na~+ current. The decrease of automaticity and excitability and prolongation of FRP are thought to be its antiarrhythmic action.